Champions League holders Barcelona will take on Arsenal in the Champions League round of 16, while Chelsea face Paris Saint-Germain.
Rafa Benitez's Real Madrid will take on Serie A side Roma, while last season's runners-up Juventus face Bundesliga giants Bayern Munich.
Manchester City take on Ukrainian team Dynamo Kiev after topping their group, while Gent, the first Belgian side to reach the last 16 since Anderlecht in 2000-01, face German outfit Wolfsburg.
Ties to be played between Feb. 16, 17; 23, 24; and March 8, 9; 15, 16:
Barca claimed European glory for a fifth time after beating Juventus 3-1 in June's final, as Luis Enrique's charges completed a memorable Treble.
Arsenal reached this campaign's knockout phase after claiming a 3-0 win at Olympiakos in the final group game to finish second, behind Bayern.
CONFIRMED: The round of 16 schedule... #UCLdraw pic.twitter.com/UZfaYXV8vu
- Champions League (@ChampionsLeague) December 14, 2015
Chelsea and PSG have been drawn together for a third season in a row, the French club having knocked out their English counterparts last term on away goals.