The Liga de Futbol Profesional (LFP) will submit a formal complaint over chants suggesting Cristiano Ronaldo "is a drunk" from some supporters at the Camp Nou during Barcelona's 5-0 win at Levante on Sunday.
News of the decision comes after the LFP said it would review videos, seen on social media, of the fans and present the evidence to the league's government-run anti-violence commission.
A statement on the official LFP website said the complaint -- which it called a "denouncement" -- was for "the shouting of chants within the stadium, during the game, in minute 65 from a group of around approximately 200 home supporters located in the lower stand behind the south goal."
It continued: "In particular, the group known as Almogavers, located with a banner identifying the group, started to sing the following chant: 'Es un borracho, Cristiano es un borracho,' in reference to the Real Madrid player Cristiano Ronaldo."
The statement said the chant had not been taken up by the rest of the crowd, whose behaviour was "completely correct during the whole game."
Ronaldo, reported to be teetotal, had been criticised for celebrating his birthday at a party that followed Real Madrid's 4-0 derby thrashing by city neighbours Atletico Madrid.
On Monday, the LFP said it wanted to apply the same yardstick used at the Bernabeu after derogatory chants toward Lionel Messi last year.
The death of a Deportivo supporter involved in fighting with Atletico Madrid fans before a La Liga match on Nov. 30 led to a push to break links between radical supporters' groups and clubs by eliminating all forms of anti-social behaviour from the game.
LFP president Javier Tebas and government ministers Jose Ignacio Wert and Miguel Cardenal all said insulting chants and songs constitute "symbolic violence" which can lead to physical violence, while the official aim is to rid stadia of all racist and xenophobic expressions.
However, it is not clear what immediate action the anti-violence commission can take against clubs in these situations.
At its most recent meeting, the body agreed to refer previous incidents of such chanting to the Spanish FA -- which has not backed the new anti-ultra measures.
The commission can, however, impose bans and fines on individuals found guilty of offences including assaulting a fellow fan, bringing fireworks into grounds, racially insulting and threatening stadium staff and urinating on stadium stairs.
Tuesday's LFP statement also said chants by Sevilla supporters during Saturday's 3-0 home win over Sevilla and by Real Betis supporters at Albacete would be referred to the anti-violence commission.
ESPN FC correspondent Dermot Corrigan contributed to this report.