Brentford suffered a shock 4-0 home defeat against League Two Oxford as seven Championship sides were ousted from the Capital One Cup on a night of first-round upsets.
The visitors ran riot in the opening stages of the game at Griffin Park as Liam Sercombe, Danny Hylton and Kemar Roofe -- the last from 40 yards -- put them 3-0 up inside the first 12 minutes, and Johnny Mullins' second-half effort completed the rout.
Along with Brentford, Championship rivals Bristol City and Huddersfield were also humbled by opposition from the fourth tier.
Bristol City went down 3-1 at Luton, for whom Jack Marriott -- with a brace -- and Paul Benson got on the scoresheet before Callum Robinson's consolation.
Huddersfield threw away the lead in a 2-1 home loss to Notts County, with Liam Noble's double turning the contest around after Murray Wallace had put the hosts in front.
Four-time champions Nottingham Forest, Bolton, Blackburn and Burnley were other major casualties, all four falling to defeat against League One opposition.
Tom Bradshaw's hat-trick, including a stoppage-time winner from the penalty spot, steered Walsall to a thrilling 4-3 triumph at Nottingham Forest while Bolton were undone at home 1-0 by Burton courtesy of Matt Palmer's late goal.
Blackburn succumbed 2-1 at home to Shrewsbury, James Collins and Tyrone Barnett notching for the away team either side of a Liam Lawrence own goal, and Burnley lost 1-0 at Port Vale where Byron Moore's late strike settled things.
Hull and Reading narrowly avoided joining the Championship exodus, both narrowly squeezing through against lower-league clubs.
The Tigers were held to a 2-2 draw at League Two Accrington Stanley and eventually secured their progress to round two via a 4-3 penalty-shootout win, while the Royals won 1-0 at League One Colchester after extra time as Chris Gunter came to their rescue.
MK Dons were also run very close, coming from behind to beat League Two Leyton Orient 2-1 at home as a Mathieu Baudry own goal and a Carl Baker effort, coming in the 90th and the 92nd minutes respectively, broke the visitors' hearts.
Wolves had to mount a fight-back as well as they overcame fourth-tier Newport 2-1 at Molineux, Ipswich did likewise as they beat Stevenage at Portman Road by the same score, while Fulham and Rotherham both edged past other League Two teams 1-0 -- Wycombe away and Cambridge at home respectively.
Kazenga LuaLua's stoppage-time goal secured Brighton a 1-0 win at League One Southend, while the other Championship-League Two contests on Tuesday all ended upset-free.
QPR won 3-0 at Yeovil, Birmingham triumphed 2-1 at Bristol Rovers, Cardiff saw off Wimbledon 1-0 at home, Charlton thumped Dagenham & Redbridge 4-1 at The Valley and Sheffield Wednesday matched that score as they defeated Mansfield at Hillsborough.
Elsewhere, there were six cases of League Two sides defeating League One opponents.
Millwall lost 2-1 at home to Barnet after extra-time, Blackpool were beaten 3-0 at Northampton, Chesterfield went down 3-1 at Carlisle after extra time, Fleetwood suffered a 1-0 home reverse to Hartlepool and Swindon were overcome 2-1 at home by Exeter.
York completed the set by winning 4-2 on penalties at home against Bradford after a 2-2 draw.
Elsewhere, Bury won 2-1 at Wigan, Sheffield United triumphed 1-0 at Morecambe, Peterborough beat Crawley 2-0 at home and Gillingham won 2-1 at Plymouth.
Rochdale and Barnsley both made it through via penalties after 1-1 draws, the former seeing off Coventry 5-3 at home in their shootout and the latter winning 7-6 at Scunthorpe.